Zhong Nanshan team: in vitro test of Lianhua Qing plague shows anti-new coronavirus effect

The latest research by Zhong Nanshan team, a member of the Chinese Academy of Engineering, suggests that Lianhua Qing plague has shown its effect son-in-vitro tests against the new coronavirus. Related paper entitled “Lianhuawen exerts anti-viral and anti-anti-inflammatory effects on new coronaviruses” Coronavirus (SARS-CoV-2) has been received by pharmacological research.

The authors include Zhong Nanshan, director of the National Key Laboratory for Respiratory Diseases of Guangzhou Medical University, and Yang Zifeng, a researcher.

Zhong Nanshan team: in vitro test of Lianhua Qing plague shows anti-new coronavirus effect

As a traditional Chinese medicine prescription, lianhua qing plague has a broad-spectrum antiviral effect and immunomodulation effect on a range of influenza viruses.

Using CPE and air spot degeneration experiments, the researchers assessed the antiviral activity of liangeer plague against SARS-CoV-2 in Vero E6 cells.

Zhong Nanshan team: in vitro test of Lianhua Qing plague shows anti-new coronavirus effect

A and B are the inhibition curves of Lianhua Qing plague and Redsey. C Air spot degeneration experiment for new coronavirus

The effects of jablin on viral body form can be seen under the transmission electron microscope.

Zhong Nanshan team: in vitro test of Lianhua Qing plague shows anti-new coronavirus effect

Ultra-thin cell slices under an electron microscope. AF is uninfected cells, BG is a new coronary infected cell, CDHI is a conjoined plague treatment cell, and EJ is a ridsewee process cell.

The expression level of protoflame cytokines after SARS-CoV-2 infection in Huh-7 cells was measured by real-time quantitative PCR assay.

Zhong Nanshan team: in vitro test of Lianhua Qing plague shows anti-new coronavirus effect

The results showed that the replication of SARS-CoV-2 in Vero E6 cells at mRNA levels significantly inhibited the replication of pro-inflammatory cytokines (TNF-alpha, IL-6, CCL-2 / MCP-1 and CXCL-10 / IP-10). In addition, the pestatoco treatment causes abnormal particle morphology of viral particles in cells.

The paper concludes that lianhua chremosignificant significantly inhibits SARS-COV-2 replication, affects viral form and plays anti-inflammatory activity in vitro. This suggests that even the plague can resist virus attacks and is expected to be a new strategy for controlling COVID-19 disease.