Restoring the world’s first new crown confirmed tiger testing process

News of the world’s first tiger infection with the new coronavirus has attracted widespread attention in recent days, said Karen A. A. , a clinical professor and director of the Animal Pathology Program at the University of Illinois School of Veterinary Medicine. Terio restored details of the tiger’s new coronavirus test in an interview with reporters on April 8. It is reported that his veterinary college completed one of the new coronavirus tests for the tiger.

Restoring the world's first new crown confirmed tiger testing process

Karen A. Terio says tigers must be anaesthetized before testing for the virus. Specific testing methods are similar to those used by humans. In this test, the researchers used the tiger’s mouth, nasal swabs or trachea to flush the sample. He stressed that testing of tigers was conducted in veterinary laboratories and did not take up testing resources in human laboratories.

On the question of whether new crown-positive animals will infect people, Karen A. Terio said in an interview that there is no evidence that cats can infect new coronavirus estovirus to humans. “The virus is much more likely to spread from person to person than to humans. “When people come to the zoo, they are far away from the cat in the zoo, so the animals in the zoo do not pose a risk to the public. “

On April 5, local time, the U.S. Department of Agriculture issued a statement confirming that a tiger at a New York zoo had been diagnosed with the new coronavirus, and several other lions and tigers had also shown symptoms of respiratory disease. Officials at the Bronx Zoo in New York say the confirmed 4-year-old female Malaya tiger Nadia and three other tigers, as well as three African lions, have both developed symptoms of dry cough and decreased appetite, but are expected to recover. Public health officials believe the animals were infected by an asymptomatic employee.

At present, the academic community is studying which animals are susceptible to the new coronavirus, especially those in close contact with humans, such as cats and dogs, are among the key subjects of study. This is of great significance to disease control.

Separate studies have shown that Cats in Wuhan are infected with the new coronavirus, which may be transmitted by humans, and that the new coronavirus replicates poorly in dogs, pigs, chickens and ducks, but is effective in ferrets and cats. The study also found that the virus can be transmitted between cats through respiratory droplets.

After confirming the transmission of cats and cats, the focus is on whether the cat is passed on. There is no evidence that cats can pass on, but this possibility has not been ruled out at a theoretical level.

Closed on March 16th, 27th shows signs of tiger disease

According to the U.S. Department of Agriculture and the Bronx Zoo, the zoo has been closed to the public since March 16, and the first tiger began to show signs of illness on March 27. There is no evidence that other animals in the zoo have symptoms of new coronavirus infection.

Four-year-old Malaya tiger Nadia began developing symptoms of dry cough in late March and was tested for the virus on April 2, said Paul Calle, chief veterinarian at the Bronx Zoo. Nadia’s sister, two Siberian tigers and three African lions have also coughed and lost their appetite, but have not yet been tested.

When Nadia initially showed symptoms of illness, the zoo’s veterinary team performed numerous diagnostic tests and blood tests. “Given the situation in New York City, of course we did a new coronavirus test,” Paul Kale said, according to National Geographic. “

After administering the sedative to Nadia, the team took samples at the zoo and sent them to Cornell University’s New York State Diagnostic Laboratory and the Veterinary Diagnostic Laboratory at the University of Illinois Veterinary Medicine for testing, before being confirmed to the National Veterinary Service Laboratory, where the results were positive.

General anaesthetic, taking samples of tiger mouth, tiger nose swab or trachea flushing

Karen A. , clinical professor and director of the Animal Pathology Program at the University of Illinois School of Veterinary Medicine. In an interview with the News Corp., Terio restored more details of the tiger’s detection.

Karen A. Terio said the new coronavirus test at the University of Illinois for Tiger Nadia “is a molecular test similar to a human test.” In the test, the researchers used a tiger’s mouth, nasal swabs or trachea to flush the sample.

For animal testing, appropriate approval is required after the sample has been taken. Although the initial detection itself does not take long, it may take additional time to approve and validate the detection. “In the United States, animals cannot be tested without regulatory approval. “

It is reported that the virus testing of Tiger Nadia was carried out by Dr. Leyi Wang’s laboratory in the vire of the Veterinary Diagnostic Laboratory at the University of Illinois.

Seven animals at the Bronx Zoo showed suspected symptoms, but only Nadia was tested. In this regard, Karen A. Terio explains that in order to test the tiger for the virus, it must first be anaesthetized. For this reason, zoo staff decided to anaesthetize and test one of the tigers they thought would get the best chance of diagnosis. “When they were anaesthetized, they didn’t know it was infected with the new coronavirus. There are many different causes of respiratory diseases in cats, so they want to make sure that other diseases can be eliminated. “

The USDA said in a statement that it gave a similar explanation. The statement said that collecting diagnostic samples from the big cat required general anaesthetic. “Since all tigers and lions exhibit similar respiratory symptoms, the attending veterinarian believes it is in the best interests of the animal to limit the potential risk of general anesthesia to one tiger. “

Conducted in a veterinary laboratory, without taking up human testing resources

After the news of The New Coronavirus was confirmed by Malay Tiger Nadia, some questioned whether testing the animals for the new coronavirus would take up human testing resources.

In response, The Bronx Zoo’s chief veterinarian, Paul Kale, said via the zoo’s official Twitter account that the new coronavirus test for Malay tiger Nadia was conducted at a veterinary school lab, unlike those used in humans. “You can’t send human samples to a veterinary lab, you can’t send animal tests to a human laboratory, so there’s no competition in this very different situation. “

“Maintaining social distance and strengthening sanitation and disinfection programmes also apply to zoos’ new crown-epidemic prevention efforts. “Karen A. Terio, who advises on the zoo’s vaccination efforts, said the zoo is continually improving and implementing preventive measures as we learn more about which species are susceptible to viral infections.

The U.S. Department of Agriculture says routine testing of zoos or animals is not currently recommended. But public and animal health officials may decide to test certain animals that show signs of disease, as well as animals known to have been exposed to the virus.

According to India Today, all zoos in India are required to monitor animal abnormalities 24 hours a day, and send samples of suspected cases every two weeks after testing positive for the new coronavirus virus in tigers at the Bronx Zoo.

New coronavirus infects Wuhan cats, and the virus can spread between cats

At present, the academic community is studying which animals are susceptible to the new coronavirus, cats are one of the key research objects.

On April 3, the research team of the National Key Laboratory of Agricultural Microbiology of Huazhong Agricultural University and the Wuhan Virus Institute of the Chinese Academy of Sciences jointly published the first study on the production of antibodies specific to the neo-coronavirus in natural conditions by animals under natural conditions, and the data showed that the new coronavirus had infected the cats in Wuhan during the outbreak (the authors said in the abstract of the paper: “Our data of sars-CoV-has2” infected cat population in Wuhan in ing outbreak. “)。 The team says the cat’s new crown infection may have been caused by a person passing the virus on to the cat. But they caution that this still needs to be verified by investigating new coronavirus infections by a large sample of cats from pre-outbreak cats. The study has not yet been peer-reviewed.

At present, there is no evidence that the new coronavirus has spread from cats to humans. However, Chen Hualan, a member of the Chinese Academy of Sciences and chief scientist of the Animal Influenza Foundation and Prevention and Control Research Innovation Team at the Harbin Institute of Veterinary Medicine of the Chinese Academy of Agricultural Sciences, published a study on March 31 in bioRxiv, and they found that a series of new coronavirus infection tests conducted in the P4 (Biosecurity Level 4) laboratory found that the new coronavirus replicates poorly in dogs, pigs, chickens and ducks, but is effective in ferrets and cats. They also found that the virus can be transmitted between cats through respiratory droplets.

The U.S. Department of Agriculture, the World Organization for Animal Health and the American Veterinary Association are all on their official websites, saying there is no evidence so far that livestock can transmit infections to humans. But they all advise sick people to take the same precautions when touching pets as they do with humans.