Science reports on the new crown of “budding killers”: super antibodies that can quickly mediat viruses

Science magazine reported online today on a new study to treat the new crown. The team, led by Professor Hsi Yi of the University of Pittsburgh School of Medicine (UPMC), has found a “nanoantibody” that not only workes as a powerful new coronavirus, but also lasts 6 weeks at room temperature! This magical weapon comes from a seemingly budding animal, the llama.

This is the third time in the last two years that a big alpaca has appeared in a top academic journal! Science magazine has described the use of alpaca antibodies to create a pan-wide influenza virus, which is expected to bring all influenza virus types to a net. In May, Cell magazine also introduced new crown-and-antibodies from alpacas.

What are the advantages of these antibodies? That’s right. The immune systems of alpacas, alpacas, and other llamas produce two types of antibodies: one is similar to a common human antibody, and the other is only a quarter the size of a normal antibody, also known as nanoantibodies. Due to its small size, nanoantibodies can be sucked directly into the lungs after atomization. In addition, these antibodies are more stable.

The big alpacas that made a major contribution to the study (Photo: Reuters; Credit: Sonya Paske, Capralogics Ltd. )

To develop nanoantibodies to the new coronavirus, the team injected a sequence of prickly proteins from a large alpaca called Wally into the new coronavirus. The professor said the name came from one of his black Labrador dogs, and that the large black alpaca used in the experiment looked very much like it.

Two months later, the alpaca’s immune system produced a batch of mature nanoantibodies. Using a mass spectrometry-based approach, the paper’s first author found the strongest nanoantibodies in Worley’s blood that bind to the new coronavirus.

The researchers then tested the antibodies’ ability to resist new crowns — less than 1 gram of antibodies per milliliter to normally infect millions of cells! By understanding the mechanisms of virus adjulytic, the researchers connected different high antiviral nanoantibodies in series and designed a series of novel nanoantibodies, trimers, to better antiviral. Numericly, the semi-inhibited concentration of its multi-price nanoantibodies is only 0.058 gram per milliliter, which is one of the most powerful meso-antibodies.

Show Yufei a 3D-printed model of nanoantibodies (purple) combined with the new coronavirus pyrethroid protein (grey) (Photo Source: Resources; Credit: UPMC)

At the same time, in collaboration with Professor Zhang Cheng of the University of Pittsburgh and ProfessorIna Schneiderman-Duhovny of the University of Hebrew in Israel, the team established a variety of molecular mechanisms for neutralization of these nanoantibodies and further modified and designed multiple point antibodies to overcome resistance caused by mutations common to virus therapy.

“Nature is the best inventor, ” the professor commented. “The technology we developed tested new crown neutral and nanoantibodies on an unprecedented scale, and we quickly found thousands of nanoantibodies with unmatched affinity and specificity. “

The researchers also point out that traditional antibodies require intravenous infusions, as well as a high demand for antibodies, so they can be costly. Nanoantibodies have an additional advantage in this regard. Researchers also expect such antibodies to be widely used to help us treat or prevent new coronary diseases and get through the outbreak early.