Nature publishes new title paper: Confirming the virus’s path into cells

Nature, the world’s top academic journal, published online a research paper on the new coronavirus (2019-nCoV) by Shi Zhengli of the Wuhan Virus Research Institute of the Chinese Academy of Sciences in the form of an “Accelerated Review Article” (Accelerated Article Preview). The paper was submitted on January 20, 2020, and was received by the journal on January 29, 2020, and published online on February 3. This is also the first time that Nature has published a study on 2019-nCoV in the form of an official paper.

Nature publishes new title paper: Confirming the virus's path into cells

Shi Zhengli’s team identified and characterized a new type of coronavirus that first caused an outbreak of respiratory disease in Wuhan, China, and revealed its similarity to severe acute respiratory syndrome (SARS) coronavirus. The team found evidence that the new coronavirus originated in bats, but the animal source of the outbreak has yet to be confirmed. The Wuhan Virus Institute also confirmed that the path of 2019-nCoV into the cells is the same as that of SARS coronaviruses, i.e. through ACE2 cell receptors.

Coronary virus has been a source of human infectious diseases, severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS) are both caused by the coronavirus. SARS-related coronaviruses are found mainly in mammals such as bats, posing a potential threat to public health.

According to the current official report, the outbreak was first detected in December 2019, and has since spread to other parts of China and overseas. Origin or in Wuhan, Hubei, China, South China Seafood Market, patients with symptoms include fever, shortness of breath and pneumonia.

As of 2400 hours on 2 February, the National Health and Health Commission had received a cumulative total of 17,205 confirmed cases (3 cases in Beijing and 1 in Jiangxi Province), 2296 cases of severe illness, 361 cumulative deaths, and 475 cases of cured discharges from the hospital, There were 21,558 suspected cases. A total of 33 confirmed cases have been reported in Hong Kong, Macao and Taiwan: 15 in the Hong Kong Special Administrative Region, 8 in the Macao Special Administrative Region and 10 in Taiwan.

Shi Zhengli’s team analyzed samples of seven patients with severe pneumonia, six of whom were workers at the Wuhan Seafood Market, which had seen its first case in December 2019.

Nature publishes new title paper: Confirming the virus's path into cells

2019-nCoV Genome

The team found that the full-length genome sequences obtained in five of the patients were almost identical to each other (more than 99.9 percent similarity), consistent with 79.5 percent of the SARS coronavirus sequences. In addition, the researchers found that the virus sequence was as similar to a bat coronavirus at the genome level of up to 96 percent, suggesting that bats may be the source of the coronavirus.

In addition, the researchers found that seven identified and sequenced non-structural proteins were also present in SARS coronaviruses, indicating that the virus was a SARS-related coronavirus, which the authors temporarily named the new coronavirus 2019 (2019-nCoV).

Nature publishes new title paper: Confirming the virus's path into cells

Molecular and serological survey of patient samples

At the same time, the team confirmed that 2019-nCoV entered the cell path in the same way as the SARS coronavirus, which is through ACE2 cell receptors. Antibodies in patients infected with 2019-nCoV show the potential to neutralise the virus at low serum dilution, but whether anti-SARS virus antibodies can cross-react with 2019-CoV still needs to be confirmed with serum from patients recovered from SARS virus infection.

The authors also developed a test that can distinguish 2019-nCoV from all other human coronaviruses.

The study also suggests that the most likely route of transmission of the virus is through the individual’s respiratory tract, although the authors note that other pathways may also require more patient data to further study the route.

It is worth noting that the Shi Zhengli team is also the source of China’s SARS virus attack team. In 2017, Shi Zhengli and others in the pursuit, research for 13 years, finally found in a small cave in Kunming, Yunnan, all the genetic composition of the SARS virus, basically completed the traceability of SARS virus. Previously, the fruit beaver, once considered the “noculprit” of SARS, was in fact an intermediate host, not the source of the virus.

Previously, on January 23rd, Ishiguro’s team was the first to publish Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans on the bioRxiv pre-plated platform And its potential bat origin reveals that Wuhan’s new coronary virus nCoV-2019 has a sequence consistency of up to 96% with a coronavirus in a bat.

According to the official website of Wuhan Virus Research Institute of the Chinese Academy of Sciences, on January 21, according to the urgent needs of the rapid disposal and emergency response in science and technology, Hubei Provincial Science and Technology Department launched the “2019 New Pneumonia Emergency Science and Technology Research Project”, set up a new type of pneumonia emergency research research expert group, Wuhan Virus Institute researcher Shi Zhengli as the group leader.